Cloning and Expression of the Pig Fast Skeletal Muscle Troponin C Gene
猪骨骼肌快肌肌钙蛋白C2基因的cDNA克隆与表达分析

A pig skeletal muscle expressed sequence tag(EST,accession number BM083186) was found to encode(amino) acid sequences with high homology to human TNNC2.In silico cloning obtained a 843 bp sequence which we named pig fast skeletal troponin C gene(TNNC2).The open reading frame of TNNC2 was between nucleotides 201-683,and was confirmed by RT-PCR.The gene encoded a 160 amino acid residue protein and had the highest similarity with human and mouse homologues,with percent identities being 93.6%,90.5%,respectively. Semi-quantitative RT-PCR analysis of mRNA from different normal tissues indicted that the TNNC2 gene was expressed in the skeletal muscle and the levels of expression were higher in the longissimus dorsi of the Duroc pig than in the same tissue of the Lantang pig.