中国鄂温克、鄂伦春、达斡尔族永生细胞系的建立与保存 Establishment and Preservation of Immortal Lymphoblastoid Cell Lines of the Ewenki,the Oroqen and the Daur Ethnic Groups in China

本文采用EBV（Epstein?Barr Virus）上清液转化B淋巴细胞，并加入环胞霉素A（Cyclosporine A）抑制T淋巴细胞，成功地对中国东北地区鄂温克族、鄂伦春族及达斡尔族的部分个体建立了永生细胞系，其中鄂温克族49株，鄂伦春族40株，达斡尔族51株，总计140株。永久保存我国特有民族的基因组，为分析其遗传学差异奠定了基础。 Abstract：The immortal lymphoblastoid cell lines were established by EBV transformation of B cells and addition of cyclosporin A to inhabit the activity of T cells.In the present study ,140 immortal cell lines of the Ewenki,the Oroqen and the Daur ethnic groups in the Northeast China were established .This is an important part of the research of human genome diversity for the exploration of the origin and progression of different ethnic groups ,and also provide enough research materials for further studies.