Establishing and Optimizing Selective Amplified DNA Fragments System Using Single Restricted Enzyme in Barley
大麦DNA单限制性酶切选择性扩增多态性技术及其优化 Establishing and Optimizing Selective Amplified DNA Fragments System Using Single Restricted Enzyme in Barley

A method named selective amplification DNA fragments (SADF) using single restrictive enzyme was established and optimized in barley. The barley genome DNA was first restricted into fragments of varied length with Pst I, Eco RI and Mse I respectively,then ligated with synthetic adapters.The ligated sets of fragments were used as templates for PCR amplification. Ideal amplification results could be obtained with different amplification procedure for Pst I and Eco RI.Except Mse I,both Pst I and Eco RI could obtain abundant and reproducible bands,but SADF of Pst I was more suitable for barley studies because of wider,more recognizable and polymorphic distribution of bands.