Structure analysis of mtDNA control region of spotted halibut (Verasper variegatus) and its related species
圆斑星鲽及相关种类线粒体DNA控制区结构分析

Spotted halibut (Verasper variegatus) is the only one species of GenusVerasper in China. The fish was naturally distributed in Yellow Sea and Bohai Sea in northern China and Kyushu in Japan and in Korean sea area. Using PCR product direct sequencing, mitochondrial control region sequences of 24 individuals of spotted halibut was confirmed and analyzed. 4 control region hyplotypes, resulting from length heteroplamy of the tandem repeat region, was obtained from these 24 fish. Sequence analysis demonstrated that there were four similar structures in the control region, i.e., extended terminal associ- ated sequences (ETAS), central conserved sequence block (CSB), conserved sequence block (CSB), and repeat region, in V.moseri, Limanda ferruginea, Reinhardtius hippoglossoides, Heppoglossoides platessoides, Paralichthys olivaceous, Solea solea, S. senegalensis, and S. lascari. By comparing with other vertebrates, we found that there were similar repeated sequences immediately after the CSB-3 in all of the anuran species.