SSLP-based Identification of Subspecies in Rice (Oryza sativa L.)
应用微卫星标记鉴别水稻籼粳亚种 SSLP-based Identification of Subspecies in Rice (Oryza sativa L.)

Six indica and japonica testers were assayed using 70 microsatellite markers.Thirty six markers distinguishing indicas from japonicas were detected.By further screening among 18 indica and japonica varieties,21 markers distributed on 12 rice chromosomes were found to be indica japonica differentiated.No indica varieties shared same patterns with any japonica varieties at 20 marker loci,of which identical patterns were observed within subspecies at 7 loci while within subspecies variations were observed at 13 loci.At the remaining locus,12 indica and 1 japonica varieties had the same allele,while other 11 japonica varieties had another allele.It also showed that SSLP was not only consistent,but also complementary,to RFLP for the subspecies identification.