A Novel Approach to Study Pathogenesis of Pathogens in vivo--Signature-tagged Mutagenesis
一种体内研究病原体致病机理的新方法——信号标签诱变技术

Signature-tagged mutagenesis (STM) is a novel approach to study pathogenesis of pathogens and to screen virulence genes with high throughput in vivo,which is based on whole genome of pathogen in question.In recent years, more than ten species of microbial pathogens have been screened with this technology. There are also unknown virulence factors being identified with exception of known virulence genes identified in all these screens. This article reviews the principle, advantages and current limitations,the requirements, modifications of STM, and to date virulence genes identified by this technology.