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遗传 2003
Studies on Taihe Silkies′Karyotype and Band Pattern
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Abstract:
This study made the chromosome slides of Taihe Silkies by the peripheral blood lymphocyte culture-drying method,and analyzed Taihe Silkies karyotype and band pattern. The results are as follow: The diploid chromosome number of Taihe Silkies was 2n=78,the basic number of chromosome arms was AF=90 and the sex chromosome type was ZZ(male symbol)/ZW(female symbol). According to the measured relative length,arm ratio and centromeric index,the first 10 pairs of macro-chromosomes are described as follows:No.1 ,9 and Z,W chromosomes were metacentrics,No.2,4,7 were submetacentrics,and No.3,6,8,10 were telocentrics. Studies on Taihe Silkies' G-band showed that the first 10 pairs of macro-chromosomes can be divided into 29 zones and 190 bands. Being treated by C-banding technique,a totally dark-stained and easily indentified W-chromosome always showed up in the female metaphase configurations. Ag-NORs were located in the short arms' telomere of No.1,2 euchromosomes and Z sexchromosome,the Ag-NORs number varied from 1-6. -NORs.