Association between single nucleotide polymorphism of ADFP gene and carcass traits and intramuscular fat content in chicken
脂肪分化相关蛋白基因多态性与优质鸡屠宰性状和肌内脂肪含量的相关性研究

In order to investigate the effect of ADFP gene on the carcass traits and intramuscular fat content of Da-heng high-quality chicken,we analyzed the coding sequence of the ADFP gene by single-strand conformation polymorphism(SSCP) and DNA sequencing in five pure lines and three crossbreds.We found three single nucleotide polymorphism sites,including A/T at base position 4 079(locus A),C/T at base position 4 843(locus B) and A/G at base position 7 070(locus C).GLM analysis for the effect of genotypes on carcass traits and intramuscular fat content demonstrated a significant effect on leg muscle percentage,abdomen fat weight,abdominal fat percentage and intra-muscular fat content in locus A(P < 0.05),a significant association with living body weight and carcass weight for locus B(P < 0.05) and a significant association with breast muscle weight and intramuscular fat content(P < 0.05),and a very significant association with breast muscle percentage for locus C(P < 0.01).It was concluded that ADFP gene may be a major gene or linked to such a gene that affects the fat traits of birds.Polymorphic loci A and C could be used as molecular markers for abdominal fat weight,abdominal fat percentage and intramuscular fat content in birds in future studies.