Discrimination of the Triticum aestivum-T. timopheevii introgression lines using PCR-based molecular markers
利用基于PCR的分子标记区分普通小麦-提莫菲维小麦渐渗系

In order to determine the fragment size of Triticum timopheevii chromosome segments introduced into wheat background and physically map the Pm6 gene, a total of 72 primers (including SSR and STS primers) were used to analyze the eight introduced introgression lines containing Pm6 gene. Referring to the available mapping information of the ana- lyzed markers on chromosome 2B, Pm6 was physically located in distal part of the long arm of chromosome 2B at the re- gion of Bin 2BL-6. The introgressed fragment sizes of the chromosome 2G in different introgression lines was determined to be as follow (from short to long): IGV1-465