Sequence Analysis of Polymorphic Fragments of Porcine H-FABP Gene
猪H-FABP基因多态片段的序列分析 Sequence Analysis of Polymorphic Fragments of Porcine H-FABP Gene

Three variant restriction sites of porcine H-FABP gene,including HinfI-RFLP in 5'-upstream, HaeIII-RFLP and HinfI*-RFLP in intron 2, were confirmed by PCR-RFLP method. The polymorphic fragments were cloned and sequenced. The results revealed a single nucleotide substitution of T-->C at position 1324 for HinfI-RFLP,C-->G at position 1811 for HaeIII-RFLP and T-->C at position 1970 for HinfI*-RFLP, respectively.