Analysis of the Splicing Site in the HMG-box of Sox8 Gene
Sox8基因HMG盒区内含子剪接位点分析 Analysis of the Splicing Site in the HMG-box of Sox8 Gene

Sox genes have diverse roles in developing processes.it was inferred there may be one intron in the HMG box by analyzing the genomic DNA sequence of the paramisgurnus dabryanus Sox8 gene.RT PCR was used to verify the intron and its splicing site.First strand of the cDNA reverse transcribed from liver RNA was amplified using the primers flanking the HMG box.RT PCR products were cloned into the pUC18 plasmid and sequenced.The comparison between the cDNA and genomic DNA sequence revealed the splicing site of the intron existing in the HMG box of the paramisgurnus dabryanus Sox8 gene.We also compared the splicing site of the intron in the HMG box of Sox8 gene between paramisgurnus dabryanus and human.These results suggest that the splicing site in the HMG box of Sox8 gene was conserved.