Structures of transgenic integration loci in transformed plants
植物转基因整合座位的结构

The plants transformed by either Agrobacterium-mediated or directly delivered DNA transformation possess complex transgenic loci.The transgenes tend to be integrated into gene-rich regions on chromosomes due to selection of transgenic cells during tissue culture rather than integrated elsewhere randomly.Fragments of transferred DNA are inter-spersed with genomic DNA and filler DNA at most of transgenic loci except a few which contain complete single-copy of transgene.Analysis of transgenic loci flanking sequences indicated that deletion,duplication and chromosomal rearrange-ment often occurred for transgene and genomic DNA.The complete transgenic structure is important for its expression in transformed plants.