Inheritance of transgene 1Ax1 in transgenic wheat (Triticum aestivum L.) with linear transgene lacking vector backbone sequence
无载体框架序列转基因小麦中外源1Ax1基因表达框的遗传分析

Transformation with gene minimal expression cassettes without vector backbone sequence is a new transformation technique. In the current research, the inheritance of the foreign 1Ax1 gene cassettes were analyzed in transgenic plants recovered with gene cassettes lacking vector backbone sequences. The results showed that linear 1Ax1 gene cassettes were stably expressed and separated at the rate of 3 to 1 in the seeds of T, generation. It suggested the functional copies of 1Ax1 gene cassettes were integrated in one sites, abiding by Mendelian genetic law. It revealed firstly the feasibility of the transformation technique with gene cassettes, and proved the inheritance of transgene cassettes in subsequent generations.