Polymorphism and Original Analysis of mtDNA D-loop of Three Leuciscus Species in Xinjiang
新疆三种雅罗鱼属鱼类mtDNA D-loop多态性及起源分化分析 Polymorphism and Original Analysis of mtDNA D-loop of Three Leuciscus Species in Xinjiang

PCR-RFLP technique was employed to amplify about 827bp of mtDNA D-loop hypervariable region of Leu-ciscus baicalensis ,L. merzbacheri and L. idus in Xinjiang. The PCR products of 56 samples with four restriction enzymes were digested:Seal, HintI,AluI,Ddel,and RFLP analysis was done then. The study indicates that all of L. species and populations have six haplotypes, L. merzbacheri has two haplotypes: BDAA, BDBA; L. baicalensis has three: AAAA, ABAA, ACAA; L. idus has one:CAAA. It is primarily considered that L. merzbacheri and L. baicalensis have more intra-population mutations. The UPGMA trees with 6 haplotypes and net genetics distance pointed out that L. merzbacheri might be original species,L. baicalensis and L. idus are evolved from L. merzbacheri.