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遗传  2001 

Application of the Non-invasive Sampling to Crested Ibises
非损伤性取样在朱?种群遗传研究中的应用 Application of the Non-invasive Sampling to Crested Ibises

Keywords: Nipponia nippon,non-invasive sampling,random,amplified polymorphic,DNA(RAPD)
朱Huan
,非损伤性取样,随机扩增多态性,DNA,鸟类种群,遗传多样性

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Abstract:

Genetic distance of sixteen crested ibises ( Nipponia nippon ) was analyzed at the DNA level by polymerase chain reaction (PCR) using random primers after DNA was sampled with non-invasive sampling. The amount of DNA was enough to apply to genetic analysis by RAPD. Eighteen primers had stable effect. The genetic distances are between 30 52% and 10.18%,which showed that the relationship between 16 crested ibises was similar. This study tried to apply a non-invasive method to obtain DNA, which is helpful to research on the genetic background of crested ibises and other tiny population based on DNA level.

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