An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves
改良CTAB法用于多年生植物组织基因组DNA的大量提取* An Efficient Macro-method of Genomic DNA Isolation from Actinidia chinensis Leaves

It is a difficult problem to isolate high quality DNA from plants containing a high contents of polyphenolics and polysaccharides, such as Actinidia plant. The protocol described in this paper is a modified CTAB (hexadecyltrimethylammonium bromide) method. High quality genomic DNA can be isolated from Actinidia plant using the improved method. The DNA is good enough for Southern blot and other uses in DNA research. The protocol is also efficient for quick and macro-DNA extraction.