Amplification of 5′cDNA End of a Disease Resistance Gene Homolog Using RACE System from Soybean
利用RACE技术扩增大豆抗病基因同源cDNA 5′末端序列 Amplification of 5′ cDNA End of a Disease Resistance Gene Homolog Using RACE System from Soybean

Based on part of a known partial cDNA sequence of a disease resistance gene homolog,KR3-1 ,obtained by screening a cDNA library from soybean, 5'-RACE-PCR was carried out with gene specific primers and universal primers. After the nested PCR reaction,an amplified fragment of 447 bp in length which overlapped the known KR3-1 sequence by 129 bp was obtained subsequently. Thus,a 5 cDNA end of KR3 was successfully cloned.