The Insertion/Deletion Polymorphism of Angiotensin I Converting Enzyme Gene and Coronary Heart Disease——Discordant Sib Pair Analysis and Transmission/Disequilibrium Test
ACE基因I/D多态与冠心病的遗传流行病学研究——表型不一致同胞对分析和传递不平衡检验 The Insertion/Deletion Polymorphism of Angiotensin I Converting Enzyme Gene and Coronary Heart Disease——Discordant Sib Pair Analysis and Transmission/Disequilibrium Test

To investigate whether the insertion/deletion polymorphism of the human angiotensin I converting enzyme gene increased the risk of coronary heart disease (CHD) in CHD pedigrees,discordant sib pair analysis (DSP) and transmission/disequilibrium test (TDT) were used.Forty-five CHD pedigrees with at least one CHD patient in the first degree relatives of probands were recruited during Oct.1998 to Feb.1999,of which parental genotype known,one or both parental genotype missing was 21,2 and 22 respectively.ACE genotype was measured by PCR technique.Conditional Logistic regression was used to analyze the DSP,and TDT-STDT program 1 1 was used for TDT and STDT.Univariable conditional Logistic regression did not find significant difference of the distribution of three differentACE genotypes in the 106 discordant sib pairs obtained from the 45 pedigrees.After adjusting effects of traditional risk factors of CHD,no significant difference of the distribution was found by multiple Logistic regression model.Neither the TDT for 13 nuclear families or STDT(sib transmission/disequilibrium test) for 24 sibships showed significant difference between the transmitted and untransmitted ACE gene D allele distributions.Our results show that the insertion/deletion polymorphism of ACE gene is not associated or linked with CHD in Chinese population.