PCR Detection of Transgenic Elements in Feed Raw Material
饲料原料中转基因成分的PCR检测 PCR Detection of Transgenic Elements in Feed Raw Material

Based on the heterogenous genes usually used in transgenic crops,the PCR technique was performed with primers derived from CaMV 35S promoter(35S-promoter,originated from cauliflower mosaic virus), NOS terminator(nopaline synthase-terminator,derived from Agrobacterium tumefaciens),EPSPS (5-enolpyruvylshikimate-3-phosphate synthase) gene,and CryIA(b) (delta-endotoxin,evolved from Bacillus thuringiensis subsp.kurstaki)gene to detect transgenic agents from feed raw materials of soybean dregs and corn gluten meal,respectively.Endogenous corn Zein (a protein extracted from corn gluten) gene,soybean Lectin (chitin-binding protein) gene and negative,positive control were applied for avoiding false results.The method established here has been succeessfully applied in detecting transgenic elements in imported feed raw material.