Measurement of Micronucleus Diameters as a Test for the Detection of Aneugens
微核直径测试作为非整倍体诱发剂的分析手段 Measurement of Micronucleus Diameters as a Test for the Detection of Aneugens

The relative diameters of micronucleus induced by colchicine(COL),Triptergium hypoglaucum(Level) Hutch(THH) and hydroquinone(HQ) were compared to evaluate their aneugenic activities in mouse bone marrow erythrocytes.Mitomycin C(MMC) was taken as the negative control in the experiment because it is a multifunctional clastogen without aneugenic potential.Diameters of the cytoplasm(D) and the micronucleus(d) of each micronucleated erythrocytes were measured with a micrometer in a microscope.The frequency of relatively large micronuclei(d>D/5) was found(71%) in COL treated group.In the THH and HQ treated groups,the relatively large micronuclei were 54% and 47%,respectively.Such micronuclei were infrequent(14%) in the MMC treated group.The results implied that THH and HQ may possess some aneugenic potential like COL.