In vitro Association of Exogenous Gene with the Nuclear Matrix of Bombyx mori Fertilised Eggs Mediated by Heterogeneous MAR
异源MAR介导外源基因与家蚕受精卵核基质的体外结合 In vitro Association of Exogenous Gene with the Nuclear Matrix of Bombyx mori Fertilized Eggs Mediated by Heterogeneous MAR

We have constructed a plasmid pMAC by inserting the core region of Antheraeayamamai fibroin gene into the BamHI site of the plasmid pCL which contains the MAR upstream huIFN-ggene. In vitro association of pCL, pAy6.8 and pMAC with the nuclear matrix of Bombyx mori fertilized eggs indicated that pMAC and pCL could tight1y bind to the nuclear mat fix, but pAy 6.8 found much more ineffectively. This provides a preliminary evidence for MAR as a vector for eukaryotic genes tranfer, integration and expression.