Localization of Sarcolectin Gene on Human Chromosome
组织凝集素在人染色体上的基因定位 Localization of Sarcolectin Gene on Human Chromosome

The localization of sarcolectin gene on human chromosome is studied. A modifled system of in situ hybridization was developed. A specific signal was generated by cmploying a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridisation buffer containing no dextran sulphate and softly denatured chromosomes. Using this system, unique sequcnce for sarcolectin assmall as 0.65kb was detectable and observed at 3q 12-13. Of 135 metaphases examined, 27(20%) had at least one grain deposited on 3q 12-13, and the backgrand was 2.81.