Detection of the Genomic DNA Polymorphisms in the Wild Crucian and Four Representative Varieties of Goldfish Using RAPD Technique
用RAPD技术检测野生鲫鱼的四个金鱼代表品种的基因组DNA多态性 Detection of the Genomic DNA Polymorphisms in the Wild Crucianand Four Representative Varieties of Goldfish Using RAPD Technique

Genomic DNA polymorphisms in the wild crucian from two different areas and in four representative varieties of goldfish were detected by using random amplified polymorphic DNA (RAPD) technique. The genomic DNA of each variety of fish was amplified with 26 primers. On average, about 134 RAPD markers were observed by each variety. The markers obtained by a single primer varied from 1 to 16. The statistical analysis of the experimental results indicated that random amplified polymorphic DNA of the goldfish and wild crucian have a high proportion of random amplified polymorphic DNA fragment shared. This further verified that goldfish is evolved from wild crucian. The cluster analysis suggested that after emerging, grass goldfish is evolved to wen goldfish, then through wen goldfish evolved to dragoneye goldfish and oval goldfish.