The Construction of A Fusion Vector Which Facilitates Protein Purification
便于产物纯化的融合表达载体的构建

A fusion cxprcssion vector plasmid pBG-2 was constructed by inserting the IgG Fc bindingdomain (FBD, 60AA) of protcin G downstream to the PRPL promoter in plasmid pBV220. There aremultiple cloning sites downstream to the FBD gene fragment, this facilitiates thc target gene fusion expression. Two spccific sitcs for chemical clcavage of protcins wcle introduccd, this makcs the purificd target protein to recovcr its native coformation SDS-PAGE showcd that the FBD was expressed up to30% of the total solub1e ccll proteins in E. coli RR l, and Western-blot displayed that the FBD had affinity toIgG.