Co-localization analysis between porcine epidemic diarrhea virus nucleocapsid protein and nucleolar phosphoprotein B23.1
猪流行性腹泻病毒核衣壳蛋白与感染细胞核磷蛋白的共定位分析

Objective]To elucidate the co-localization characteristic between porcine epidemic diarrhea virus(PEDV) N protein and B23.1 phosphoprotein.Methods]Two pairs of primers used to amplify N gene and B23.1 gene were designed and synthesized according to CV777 N gene sequence(AF353511) and human nucleolar phosphoprotein B23.1 gene sequence(BC050628.1),respectively.The PEDV N gene and B23.1 gene were amplified by RT-PCR from PEDV strain CV777 and Vero E6 cells,respectively;then cloned into eukaryotic expression ...