Diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang
黑龙江大豆胞囊线虫胞囊可培养细菌多样性

Objective] To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province.Methods] Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences.Results] Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences.In total 7 genera and 22 species were identified,including 46 strains in Gammaproteobacteria(51.1%),32 in Firmicutes(35.6%),10 in Betaproteobacteria(11.1%),and 2 in Alphaproteobacteria(2.2%).The dominant bacteria species were Pseudomonas and Bacillus.Conclusion] There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang,and these bacteria may play a physical and ecological roles in nematodes.