Inter simple sequence repeat analysis for Pleurotus cornucopiae
白黄侧耳Pleurotus cornucopiae微卫星间区(ISSR)分析

The anchored ISSR analysis of Pleurotus cornucopiae was carried out for identification of the thirty cultures cultivated from 1982 to 2004 in China. The polymorphic DNA fragments could be produced by both of the primer P4 and P5 for the tested cultures of P. cornucopiae. Forty-five bands arose from primer P4, 200~20000 bp in length and thirty-nine bands arose from primer P5, 500~15000 bp in length. All of the bands brought polymorphic among the tested strains. The thirty cultures of P. cornucopiae were divided into 15 groups,i.e.15 stains under similarity of 61% by cluster analysis.The cultures with the same ISSR profiles and 100% similarity of cluster analysis probably belong to the same strain. The results show that the environment of the wild mushrooms is faced with the contamination of germplasms from the commercial varieties. The ISSR profiles of wild P.cornucopiae in Hebei,Shandong and Yunnan are equal to those of commercial varieties cultivated before,and the similarity analyzed by UPGAMA is 100%.