Purification and characterization of mannanase from an alkaliphilic mannanase producing bacterium HMTS15
一株产甘露聚糖酶菌株的分离鉴定及酶的纯化与性质

Objective] To isolate and identify an alkaliphilic mannanase producing bacterium,purify and characterize mannanase thereof.Methods] Mannanase-producing alkaliphilic bacterium HMTS15 was isolated by alkaline agar with konjak from water sample of Hamatai Lake in Inner Mongolia,China.The morphological,biochemical and physiological characteristics and 16S rRNA gene were analyzed to identify the taxonomic position of strain HMTS15.Mannanase produced by strain HMTS15 was purified by four steps including(NH4)2SO...