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微生物学报 2011
Identification of extracellular polysaccharide-associated genes in Xanthomonas oryzae pv. oryzicola
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Abstract:
Objective] Previously,seventeen extracellular polysaccharide-associated mutants of Xanthomonas oryzae pv.oryzicola(Xoc) were acquired from our randomly Tn5-inserted mutant library.To know the Tn5-inserted genes of these mutants and their contribution to EPS production and virulence in rice,Methods] in this study,we first identified and characterized the Tn5-targeted genes in these mutants and then inoculated them in susceptible rice for virulence assessment.Results] Tn5 transposon was inserted in genes o...