Genetic engineering of Pichia pastoris expression system for improved secretion of heterologous proteins--a review
改良毕赤酵母分泌表达外源蛋白能力的研究进展

Due to the potential for producing soluble,correctly folded protein with high yield,Pichia pastoris is currently one of the most effective hosts for the expression of heterologous proteins.However,limitations of expression efficiency are often reported for many different heterologous proteins.Accumulating evidences suggest that protein folding and processing of heterologous protein is a major bottleneck during secretion process in yeast.Apart from optimization of the fermentation process,the current strateg...