Diversity of culturable bacteria in the typical frozen soil areas in China
中国典型冻土区土壤可培养细菌多样性

Objective] The diversity of culturable bacteria in the six frozen soils from High-latitude area and High-altitude area were analyzed by using culture-dependent approaches.Methods] Three solidified media were used to isolate culturable bacteria.Bacterial 16S rRNA genes of the isolates were PCR amplified using bacteria-universal primers,and then were sequenced.The resulting bacterial 16S rRNA gene sequences were subjected to phylogenetic analysis.Results] The abundance of culturable bacteria ranged from 4....