Analysis of xylanases derived from the metagenomic BAC clone library of yak rumen
牦牛瘤胃元基因组文库中木聚糖酶基因的分析

Objective] The diversity of rumen microbial xylanases and their degradation characteristics were studied and the resources of new xylanases genes and enzymes were provided.Methods] According to the result of the high-throughput sequencing of the rumen microbial metagenome bacterial artificial chromosome(BAC) clones library,the diversity of xylanase genes was analyzed and screened.Then one xylanase and its downstream xylosidase gene screened was cloned and expressed in Escherichia coli.The enzyme character...