Immunogenicity of attenuated Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae in mice
表达猪肺炎支原体免疫原性基因的重组猪霍乱沙门氏菌对小鼠的免疫原性

Objective]The study was carried out to construct and characterize Salmonella choleraesuis vaccine strain expressing immunogenic genes of Mycoplasma hyopneumoniae and to test its immunogenicity in mice.Method] We made p36,p46,p65 and p97R1-Nrdf,the main immunogenic genes of Mycoplasma hyopneumoniae,to insert into the prokaryotic expression plasmid pYA3493.Then these recombinant plasmids and pYA3493 were electroporated into C500 asd-mutant,resulting in the recombinant Salmonella choleraesuis vaccine strains...