Cholesterol-degrading by Lactobacillus plantarum LpT1 and LpT2 in vitro
植物乳杆菌LpT1和LpT2体外降胆固醇机制

Abstract: Objective] To explore the mechanism of cholesterol-degrading by Lactobacillus plantarum LpT1 and LpT2 in vitro. Methods] After Lactobacillus plantarum LpT1 and LpT2 being inoculated and cultured in the medium MRS, MRS+CH, MRS+CH+S and MRS+CH+N, the cholesterol content in supernatant, deposit and cells, and the total cholesterol content before and after inoculation were determined and compared to primarily predict the mechanism of cholesterol-degrading by L. plantarum. Results] The mechanism of cholesterol- degrading by L. plantarum LpT1 and LpT2 in vitro involved in metabolic and non-metabolic pathway. Non-metabolic pathway was related to co-precipitation and cell absorption. Metabolic pathway was due to the production of the special enzymes during the growth of L. plantarum which made the cholesterol degrade to be other materials and caused its content reduction. Conclusion] L. plantarum has shown its ability to degrade cholesterol in vitro.