Soil bacterium Pseudomonas sp. 593 synthesizes phosphatidylcholine via phosphatidylcholine synthase pathway
土壤假单胞菌593使用Pcs途径合成磷酯酰胆碱

Objective] Prokaryotes synthesize phosphotidylcholine by using phospholipid N-methylation or phosphatidylcholine synthase pathway or both.To confirm which pathway the soil bacterium Pseudomonas sp.593 utilizes,we tested its phosphotidylcholine synthesis,cloned the pcs gene encoding phosphatidylcholine synthase,examined Pcs activity,and constructed a pcs-mutant.Methods] To clone the pcs gene from Pseudomonas sp.593 genomic DNA,we firstly aligned amino acid sequences of phosphatidylcholine synthases in diff...