Complete sequence of a full-length DNA and molecular characterization of one plasmid from chinaberry (Melia azedarach Z) witches''-broom phytoplasma
苦楝丛枝植原体质粒的测定与分子特征

Objective] To clone plasmid from chinaberry witches'-broom phytoplasma and analyse its molecular characterization.Methods] Fragments of one plasmid(pCWBFq) in chinaberry witches'-broom phytoplasma-Fuqing strain(CWBFq) were amplified with primer pairs which were designed according to plasmid sequences published on NCBI.Transmembrane domain and subcellular localization predictions of proteins encoded by the plasmid pCWBFq as well as phylogenetic analysis among the plasmid sequences were completed by using b...