Isolation and HPLC-HRMS analysis of anti-tumor constituents from mycelia of a strain of Hirsutella sp.
一种被毛孢菌丝体中抗肿瘤活性组分的分离纯化和液质联用分析

During a screening of fungal strains with anti-tumor activity against CHO cells, a strain named RCEF0851 of Hirsutella sp. whose extract exhibited significant bioactivity was found. Extraction tests with different solvents revealed that acetylacelate was the best solvent for obtaining the extraction with active components. The extract can be best purified by using RP column Synergi Hydro. Bioactive guided isolations resulted in obtaining a pure active compound Hh-1. The inhibition rate of the compound against CHO cells reached 83.61% at the concentration of 40μg/mL. HPLC-HRMS analysis revealed that the possible molecular formula of the active compound was C30H22O10 which was isolated from entomogenous fungi for the first time.