Construction of cheA insertion mutant of Campylobacter jejuni and the alteration in coloning of mice
空肠弯曲菌cheA基因插入突变及其对小鼠空肠定植能力的影响

Objective]To construct cheA(chemotaxis,che) insertion mutant of Campylobacter jejuni and to observe the role of cheA gene in adhesion of Campylobacter jejuni on mice jejunum.Methods]We generated cheA gene insertion mutant of C.jejuni NCTC11168 based on homologous recombination.The cheA mutant was checked by PCR and sequencing.We detected the difference in mice jejunal adhesion between cheA mutant and wild-type of C.jejuni NCTC11168 by CFU(Colony-Forming Units) counting of C.jejuni in jejunal content.We co...