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OALib Journal期刊
ISSN: 2333-9721
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Evidence of survival and sustainable control on pests of Beauveria bassiana after artificial release in Massion pine plantation
球孢白僵菌释放后在森林系统中定殖及持续控虫作用的证据

Keywords: Inoculative release,population,intron,28S rDNA
接种式放菌
,种群,Ⅰ型内含子,28S,rDNA

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Abstract:

In a Massion pine plantation ,non-woven fabric bands impregnated with Beauveria bassiana were used inoculatively against the pine sawyer, Monochamus alternatus. Cadavers of different insects were collected before and after the fungal release ,and 28S rDNA group I intron markers were used to detect recovery of the released strain. Among 87 isolates collected after release ,66 were identical to the released strain , accounting for 75.86%. In 6 months after release , recovery of the released strain varied withom 75%-90.9%;with an average of 81.74%.Thus,the released strain colonized in the pine ecosystem and provided sustainable sawyer control.Population structure changed after the release,and the released strain took over the dominant position originally by indigenous type isolates.

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