|
微生物学报 2011
Gene deletion and functional analysis of the EAL domain protein VieAxoo in Xanthomonas oryzae pv. Oryzae
|
Abstract:
Objective]To better reveal the functions of key members involved in cyclic di-GMP signal metabolism pathways in the bacterial blight pathogen of rice Xanthomonas oryzae pv.oryzae (Xoo).Methods]vieAxoo (PXO _ 04753),a gene putatively encoding the EAL domain proteins was investigated by gene deletion mutation using the marker exchange,complementation and phenotypic analysis.Result]The sequence of vieAxoo cloned from genomic DNA of the wild-type strain PXO99A was found to be highly conserved in plant-pathog...