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ISSN: 2333-9721
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Gene deletion and functional analysis of the EAL domain protein VieAxoo in Xanthomonas oryzae pv. Oryzae
水稻白叶枯病菌EAL结构域蛋白VieAxoo基因缺失突变和功能分析

Keywords: Keywords: Xanthomonas oryzae pv,oryzae,c-di-GMP,vieAxoo,gene deletion,functional characterization
关键词:水稻白叶枯病菌
,环鸟苷二磷酸,vieAxoo,缺失突变,功能鉴定

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Abstract:

Objective]To better reveal the functions of key members involved in cyclic di-GMP signal metabolism pathways in the bacterial blight pathogen of rice Xanthomonas oryzae pv.oryzae (Xoo).Methods]vieAxoo (PXO _ 04753),a gene putatively encoding the EAL domain proteins was investigated by gene deletion mutation using the marker exchange,complementation and phenotypic analysis.Result]The sequence of vieAxoo cloned from genomic DNA of the wild-type strain PXO99A was found to be highly conserved in plant-pathog...

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