Comparative study of the promoter activity of metabolism-related pabB and lpdA genes between Mycobacterium tuberculosis strains H37Rv and H37Ra
结核分枝杆菌H37有毒株与无毒株的代谢相关基因pabB和lpdA启动子活性分析

Objective Comparative genomic study revealed mutations at the promoter regions of pabB and lpdA in avirulent Mycobacterium tuberculosis strain H37Ra compared to its virulent counterpart strain H37Rv.We used LacZ reporter system to test whether those mutations will affect promoter activity and its potential relationship to virulence attenuation of H37Ra.MethodsPromoter regions of pabB and lpdA were predicted by theNeural Network Promoter Predictionmethod(http:/ /www.fruitfly.org /seq_tools /promoter.html).Pr...