Prokaryotic expression of Glutamyl tRNA Synthetase of Streptococcus suis and its immunization experiment
猪链球菌谷氨酰胺tRNA合成酶的原核表达产物的小鼠免疫试验

Objective]Glutamyl tRNA Synthetase (Gts) is a protease which catalyzes esterification between tRNA and glutamine. The immunogenicity of Gts was evaluated through immunization and challenge experiment. Methods and results] We cloned gts from the genome of SC19,and inserted it into prokaryotic expression plasmid pET28a-gts. The recombinant vector was transformed into E. coli BL21. Induced by IPTG,one 58 kD protein,was expressed and purified by using Ni~+-NTA column (Novagen). The purity of rGtS was 93.3% an...