Construction of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and the expression of vitreoscilla hemoglobin
委内瑞拉链霉菌秦岭变种属间接合转移系统的构建及透明颤菌血红蛋白的表达

Abstract:Objective] To Construct of conjugal transfer system of Streptomyces venezuelae var. Qinlingensis and express of vitreoscilla hemoglobin.Methods] Intergeneric genetic transfer system was based upon plasmid pSET152 and pHZ1358 from Donor E.coli ET12567(pUZ8002) to Streptomyces venezuelae var. Qinlingensis Results] Our data showed that Intergeneric genetic transfer system was demonstrated and optimized. Integrating plasmid pJD100 containing Perm Eand construction gene of vhb, constructed by SOE-PCR, was transformed to ET12567(pUZ8002), and then transfered to Streptomyces venezuelae var. Qinlingensis by conjugation. The integrating expression of vhb gene in Streptomyces venezuelae var. Qinlingensis was verified by PCR and CO binding diference spectrum. Couclusion] In summary, intergeneric genetic transfer system was demonstrated and optimized, and this is the first report to express vhb gene in Streptomyces venezuelae var. Qinlingensis.