Antifungal Activities and Stability of Extracts from Culture Liquid of Hypoxylon perforatum to Sphaeropsis sapinea
炭团菌提取物对樟子松枯梢病菌的抑菌活性及稳定性

Objective]Our research objective is to obtain the active substances from culture liquid 0f Hypoxylon perforatum withinhibitory effect on Sphaeropsis sapinea growth and germination.Methods]Water and ester were used for extracting activesubstances from culture liquid of Hypoxylon pefforatum,either by extracting directly from cultural liquid,or extracting underultrasonic.Growth-inhibiting rate and germination-inhibiting rate were used as the index of antifuIlgal activity.Results]Fiveextracts from culture liquid of Hypoxylon pefforatum showed antifungal activities to Sphaeropsis sapinea growth and germination.Conclusion]Ethyl acetate-extract from culture liquid of Hypoxylon perforamm has a highly antifungal activity to Sphaeropsissapinea,hish stability in the natural environment.So it has a highly development of the value and application prospect.Themainly inhibiting active substance is methyl p-methoxy cimaamate.which as medicine intermediate was used in cosmetic to be UV-protection absorbefacient.Diisobutyl phthalate and Dibutyl phthalate are all the oolllnlon plastic-enhancer for PolyvinylChloride(PVC).It is very important in medicinal industry and chemistry industry to discovering the natural presence of the threecompounds.