Contributions of putative lipoate-protein ligase to the virulence of Streptococcus pneumoniae
假想的脂蛋白连接酶对肺炎链球菌毒力的影响

Abstract: Objective] To investigate the effect of putative lipoate-protein ligase (LPL) on the virulence of Streptococcus pneumoniae. Methods] lpl gene deficient strain was constructed by LFH-PCR and identified by PCR and sequencing. The cell adherence assay and mice challenge assay were used to observe the differences between wild strain and the mutant in the pathopoiesis of Streptococcus pneumoniae. Results] Mice virulence experiments showed that the median lethal time of wide type and the lpl mutant are both 12h, no statistics difference ;The ability of adherence of the mutant was greater than the wild strain( P < 0.01); The capsule stain in-vivo showed that the wild strain and the mutant both had the capsule. Conclusion] lpl gene inhibits the adherence to host, but no affect on the ability to infection mice by intraperitoneal injection.