PCR Identification of Escherchia coli F18 and It''''s Subtypes
大肠杆菌F18菌毛及其亚型的PCR鉴定

The fimbrial adhesin F18 is frequently found on the surface of enterotoxigenic Escherichia coli (ETEC) and vero- toxigenic E. coli (VTEC) strains which are responsible for diarrhea and edema disease in weaned piglets, and it have been implicated as possible virulence factors that mediated bacterial colonization on small intestinal epithelial cells. The F18 adhesin occurs in two serological variants, F18ab and F18ac. Three synthetic oligonucleotide primers derived from the fedA gene of F18ab fimbriae were used in polymerase chain reaction (PCR) amplification procedure to detect some enteric pathogens, such as F18ab~ strains, F18ac~ strains, K88~ strains, K99~ strains, 987P~ strains and F41~ strains. The results showed that the established PCRs were not only able to identify F18~ E. coli strains, but also to distinguish F18ab~ and F18ac~ strains.