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微生物学报 2008
ISSR fingerprint analysis and SCAR marker of major cultivated strains of Auricularia auricula in China
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Abstract:
Inter-simple sequence repeat(ISSR) was used to conduct DNA fingerprint analysis of thirty-four major cultivated strains of Auricularia auricula in China, and these strains primarily conducted a standard DNA fingerprint. On the base of ISSR analysis, the 34 strains were clustered by UPGMA and two specific ISSR bands from strain 173 and 186 were converted into two sequence characterized amplified region (SCAR) markers which were used to rapid strain identification. The result showed that the genetic background of cultivated strains of A. auricula was similar and nomenclature of the cultivated strains was confused and full of synonyms. Utilization of ISSR fingerprint and its SCAR marker for rapid identification of cultivated strains in A. auricula is practicable and significative.