Sequence analysis of the internal transcribed spacer of gene coding for rDNA in Russula subnigricans and R. nigricans
亚稀褶黑菇和稀褶黑菇的ITS序列分析

The internal transcribed spacer of gene coding for rDNA of Russula subnigricans and Russula nigricans were sequenced and analyzed, and 5.8S rDNA sequences and ITS1 sequences of R. subnigricans were reported for the first time. The results show that there is no difference between 5.8S rDNA sequences of R. subnigricans and R. nigricans, but the length discrepancy and sequence polymorphism of ITS1 and ITS2 are existed between the two species. Their intraspecies distinctions did not exceed 5%, but their interspecies distinctions were about 10%. The ITS sequence can be used as identification method for R. subnigricans and R. nigricans.