Construction of T-DNA insertion mutants of Botrytis cinerea via Agrobacterium tumefaciens mediated transformation and sequence analysis of insertion site
农杆菌介导的灰葡萄孢T-DNA插入突变体库构建及插入位点分析

Objective] Construction of Botrytis cinerea T-DNA insertion mutant library through Agrobacterium-mediated transformation and for further understanding of the molecular mechanisms underlying the pathogenicity of Botrytis cinerea.Methods] Agrobacterium containing the binary vector pCMBIA1390 was used for transformation of Botrytis cinerea.Hygromycin resistant transformants were screened out and subjected to biological and morphological observation.Detached tomato leaves were used for pathogenicity assay.The...