Bacterial diversity in a hot gas spring soil of MaNasi County, Xinjiang by culture-independent approach
新疆玛纳斯热气泉免培养土壤细菌多样性分析

Objective]In order to investigate bacteria diversity in a hot gas spring soil of MaNasi county,Xinjiang.Methods]Total DNA were directly extracted from the soil of a hot gas spring from Xinjiang.16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned.Positive clones were identified by restriction fragment length polymorphism(RFLP),and unique rDNA types clones were sequenced,analysed and then constructed phylogenetic tree.Results]Twenty-nine oper...